Catalogue number: NB-79-0001

Features
▪ Detects double-strand DNA and single-stranded RNA effectively.
▪ Offers a safe alternative to ethidium bromide staining.
▪ Exhibitssensitivity on par with EtBr.
▪ Non-toxic, non-mutagenic, and non-carcinogenic composition.
▪ Produces no hazardous waste, ensuring environmental friendliness.

Description

NeoDye DNA Orange is a sensitive, stable and relatively safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide
(EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. The sensitivity of NeoDye DNA Orange is much higher than EB,
and destaining is not required. NeoDye DNA Orange and EB have virtually the same spectral properties.

Storage

Atroom temperature protected from light fortwo years

Highlights

Nontoxic: Unique lipophilic and macromolecular properties of NeoDye DNA Orange make it incapable of penetrating cell membranes.
Ames test also shows the mutagenicity of NeoDye DNA Orange is far less than that of EB.
• Highly sensitive: Suitable for staining fragments of different sizes in electrophoresis gel.
• Highly stable: Suitable for using microwave or other heating methods to prepare agarose gel; extremely stable at room temperature
under acidic or alkaline conditions; highly resistant to light.
• High signal-to-noise ratio: Strong fluorescence signal of the sample with low background signal
• Simple operation: Similar to ethidium bromide, the dye does not degrade during the process of preparing gel or electrophoresis. It
only takes 30 minutes for staining after electrophoresis, and the fragment can be visualized by a UV-transilluminator directly without
detaining or washing.
• A broad range of applications: Applicable for precast protocol (add dye during gel preparation) and post-stain protocol (submerge
the gel in the staining solution); suitable for agarose gels and polyacrylamide gels electrophoresis; suitable for staining dsDNA, ssDNA
or RNA.
• Changing the existing imaging system is not required: Suitable for both standard EB filter and SYBR filter; same with the standard
UV-transilluminator used for EB staining observation; optimal excitation in the UV region around 300 nm.

Components

FOR RESEARCH USE ONLY