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Mouse Monoclonal Anti-Adipophilin / Perilipin-2 (Marker of Obesity) [Clone ADFP/1494]
Cat# NB-36-00077-P1ABX
Size : 100ug
Brand : Neo Biotech
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Adipophilin / Perilipin-2 (Marker of Lipid Accumulation) Antibody [ADFP/1494]
Catalog Number Formulation Size Price
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Adrenal stained with Adipophilin Mouse Monoclonal Antibody (ADFP/1494).
SDS-PAGE Analysis Purified Adipophilin Mouse Monoclonal Antibody (ADFP/1494). Confirmation of Integrity and Purity of Antibody.
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Lipid droplet, Membrane
Gene Name
PLIN2
Positive Control
Adrenal gland or Cerebellum., HepG2 or JAR cells. Liver
Immunogen
Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein (exact sequence is proprietary)
Alternate Names
Perilipin-2, Adipophilin, Adipose differentiation-related protein, Adipophilin; ADFP; Adipose differentiation-related protein (ADRP); Perilipin-2 (PLIN2)
Functions
- Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
Key References
- Kern, P.A., et al. 2004. Perilipin expression in human adipose tissue is elevated with obesity. J. Clin. Endocrinol. Metab. 89: 1352-1358
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/9003395/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/21269460/
- https://pubmed.ncbi.nlm.nih.gov/22223895/
- https://pubmed.ncbi.nlm.nih.gov/22814378/
- https://pubmed.ncbi.nlm.nih.gov/23186163/
- https://pubmed.ncbi.nlm.nih.gov/24275569/
- https://pubmed.ncbi.nlm.nih.gov/26357594/
- https://pubmed.ncbi.nlm.nih.gov/34077757/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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