Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG3 monoclonal antibody, clone RM218 (Cat # MAB12770) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both mouse IgG3 (kappa) and IgG3 (lambda); No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
- Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against mouse IgG3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse IgG3.
Sequence
N/A
Reactivity
Mouse
Form
Liquid
Conjugation
Biotin
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.005-0.2 ug/mL)
Flow Cytometry
Immunocytochemistry (0.5-2 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
Immunoprecipitation
Western Blotting (0.1-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG3 monoclonal antibody, clone RM218 (Cat # MAB12770) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both mouse IgG3 (kappa) and IgG3 (lambda); No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Flow Cytometry
- Gene Info — AI324046