Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/20 - 1/80, depending on the method used.
Reactivity
Mouse
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Pools of purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity
The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against mouse serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgE used as immunogens. It does not react with IgG including all subclasses, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also react with membrane-bound IgE in peripheral blood cells as tested by immunofluorescence microscopy. Cross-reactivity: This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this conjugate has not been tested in detail
Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Shipping
Ambient
Note
Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies crossreacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
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