Mouse Anti-P4HA1 Monoclonal Anibody (Clone : 4F1)

Cat# FNab06079

Size : 100ug

Brand : FineTest

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Synonyms:Prolyl 4-hydroxylase subunit alpha-1 (4-PH alpha-1)|Procollagen-proline antibody, 2-oxoglutarate-4-dioxygenase subunit alpha-1|P4HA1|P4HA antibody
Catalogue No.:FNab06079Reactivity:Human, Mouse, Rat
Host:MouseTested Application:ELISA, WB, IHC, IF
Clonality:monoclonalIsotype:IgG1
Size
100µg Inquiry
Dispatch Time: About 3 working days
Product Name
P4HA1 antibody
Catalogue No.
FNab06079
Size
100μg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Clonality
monoclonal
Isotype
IgG1
Clone ID
4F1
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
prolyl 4-hydroxylase, alpha polypeptide I
Alternative Names
Prolyl 4-hydroxylase subunit alpha-1 (4-PH alpha-1)|Procollagen-proline antibody, 2-oxoglutarate-4-dioxygenase subunit alpha-1|P4HA1|P4HA antibody
UniProt ID
P13674
Observed MW
61 kDa
Application
Tested Applications
ELISA, WB, IHC, IF
Recommended dilution
WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:50-1:500
Validated Images
PC-3 cells were subjected to SDS PAGE followed by western blot with FNab06079(P4HA1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab06079(P4HA1 Antibody) at dilution of 1:500 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed PC-3 cells using FNab06079 (P4HA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)
Background
Catalyzes the post-translational formation of 4-hydroxyproline in-Xaa-Pro-Gly-sequences in collagens and other proteins.

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