MHC Class 2, DQ, DR, Polymorphic (MHC Class II) (PE)

Cat# M3887-43B-100T

Size : 100Tests

Brand : US Biological

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M3887-43B MHC Class 2, DQ, DR, Polymorphic (MHC Class II) (PE)

Clone Type
Polyclonal
Host
mouse
Source
sheep
Isotype
IgG1
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Gt Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain.||Applications:|Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul|Optimal dilutions to be determined by the researcher.||Hybridoma: |NS1 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 28.1|Host: mouse|Source: sheep|Concentration: ~100 Tests/ml (after reconstitution)|Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Sheep alveolar macrophages.|Specificity: Recognizes a polymorphic epitope on sheep MHC class II DQ and DR molecules. Recognizes sheep MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1. Species Crossreactivity: goat and bovine||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Sheep alveolar macrophages.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes a polymorphic epitope on sheep MHC class II DQ and DR molecules. Recognizes sheep MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1. Species Crossreactivity: goat and bovine