iTAg Tetramer/APC - H-2 Kb LCMV (LEYDFNKL)

Cat# TB-5014-2

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TB-5014-2
Tetramer
50 tests

DATA SHEET

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 81

TargetLCMV
Product TypeTetramer
ApplicationFCM
ConjugateAPC
ClassClass I
Species ReactivityMouse
AlleleH-2Kb
Peptide SequenceLEYDFNKL
Research AreaImmunology, Immune Monitoring
ProtocolsFCM
SpecificityL protein
Storage Temperature4°C
Regulatory StatementFor Research Use Only. Not for use in diagnostic procedures.
TB-5014-2
Tetramer
50 tests