IP3(Inositol Triphosphate) ELISA Kit

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Cat# E-EL-0059-96T

Size : 96T

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reacitivity	Universal
Specificity	This kit recognizes Universal IP3 in samples.No significant cross-reactivity or interference between Universal IP3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal IP3. During the reaction, Universal IP3 in the sample or standard competes with a fixed amount of Universal IP3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal IP3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal IP3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Background

Inositol-1, 4-bisphosphate (Ins 1-(1, 4)P2) and inositol-1, 4, 5-triphosphosate (IP3). They are derived in the body from enzymatic hydrolysis of phosphatidyl inositol substances (e.g., phospholipase C). So far, nearly 20 different phosphoinositols have been found in cells, many of which play an important role as information substances. The most important is inositol triphosphate (IP3), which is the enzymolysis of triphosphoinositide. Its main effect is to induce the release of Ca2+ from the intracellular reservoir and instantly increase the concentration of Ca2+ in the cellular fluid. This process is based on the fact that Ca2+ is a widespread intracellular messenger, so it plays an important regulatory role in cellular response, and it is complementary to the regulatory effect of diacylglycerol. Mainly used as biochemical reagent. Inositol-1, 4-bisphosphate (Ins 1-(1, 4)P2) and inositol-1, 4, 5-triphosphosate (IP3). They are derived in the body from enzymatic hydrolysis of phosphatidyl inositol substances (e.g., phospholipase C). So far, nearly 20 different phosphoinositols have been found in cells, many of which play an important role as information substances. The most important is inositol triphosphate (IP3), which is the enzymolysis of triphosphoinositide. Its main effect is to induce the release of Ca2+ from the intracellular reservoir and instantly increase the concentration of Ca2+ in the cellular fluid. This process is based on the fact that Ca2+ is a widespread intracellular messenger, so it plays an important regulatory role in cellular response, and it is complementary to the regulatory effect of diacylglycerol. Mainly used as biochemical reagent.

Research Area

Biochemicals