Interleukin 1 Beta (IL1b) BioAssay™ ELISA Kit (Canine)

Interleukin 1 Beta (IL1b) BioAssay™ ELISA Kit (Canine) is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1b in canine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.||Detection Range:|7.8-500pg/ml||Sensitivity:|2.9pg/ml||Intra-Assay CV:|<10% ||Inter-Assay CV:|<12%||Kit Components:|*026025A: Microtiter Strips, 1x96 wells, Pre-coated; ready to use|*026025B: Standard, 2x1vial |026025C: Standard Diluent, 1x20ml|*026025D: Detection Reagent A, 1x120ul |*026025E: Detection Reagent B, 1x120ul |026025F: Assay Diluent A, 1x12ml|026025G: Assay Diluent B, 1x12ml|026025H: TMB Substrate, 1x9ml|026025K: Stop Solution, 1x6ml|026025L: Wash Buffer, 30X, 1x20ml ||Precaution:|The Stop Solution (026025K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.||Storage and Stability:|Store *026025A, *026025B, *026025D and *026025E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.||Assay Procedure Summary:|1. Prepare all reagents, samples and standards.|2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.|3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.|4. Aspirate and wash 3 times.|5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.|6. Aspirate and wash 5 times.|7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.|8. Add 50ul Stop Solution. Read at 450nm immediately.