IgM, Human Fc5u (FITC)

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Cat# I1903-38A-1mg

Size : 1mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

Contact local distributor :

Phone : +1 850 650 7790

I1903-38A IgM, Human Fc5u (FITC)

Clone Type

Polyclonal

Host

goat

Source

human

Isotype

F(ab’)2

Grade

Affinity Purified

Applications

FLISA IF

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications: |Suitable for use in FLISA and Immunofluorescence. Other applications have not been tested.||Recommended Dilution: |Optimal dilutions to be determined by the researcher.||Absorption Wavelength: |495 nm ||Emission Wavelength: |528 nm||Storage and Stability:|Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C.|Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

Product Type: Pab|Isotype: F(ab’)2|Host: goat|Source: human|Concentration: ~1mg/ml (after reconstitution) (by UV absorbance at 280 nm)|Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.05% Sodium Azide, 0.01% Thimerosal. Reconstitute with 1ml sterile ddH2O.|Purity: Purified by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |Immunogen: Human IgM Fc5µ fragment|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against|other human heavy or light chain isotypes||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human IgM Fc5µ fragment

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.05% Sodium Azide, 0.01% Thimerosal. Reconstitute with 1ml sterile ddH2O.

Purity

Purified by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against|other human heavy or light chain isotypes