IgA (AP)

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Cat# I1890-05C-1mg

Size : 1mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

Contact local distributor :

Phone : +1 850 650 7790

I1890-05C Rabbit Anti-IgA (AP)

Clone Type

Polyclonal

Host

goat

Source

human

Isotype

IgG

Grade

Affinity Purified

Applications

DB E IHC WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) ||Application(s): suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. ||Recommended Dilution(s): This product has been assayed against 5.0 ug of Human IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. ||Storage Conditions: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

Applications

Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: ~1mg/ml (UV280)|Form: Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).|Purity: Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |Immunogen: Human IgA heavy chain|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against Human IgG F(c). Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human IgA heavy chain

Form

Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).

Purity

Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against Human IgG F(c). Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.