HyperPFU™ high-fidelity DNA polymerase is composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, it produces PCR products with very high speed and accuracy. Also, HyperPFU™ high-fidelity DNA polymerase is tolerant of various inhibitors, allowing for robust amplification with minimal optimization. HyperPFU™ high-fidelity DNA polymerases is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. It becomes the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.
HyperPFU™ high-fidelity DNA polymerase is important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. HyperPFU™ high-fidelity DNA polymerases is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), it is one of the most accurate thermostable polymerases available. It possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
HyperPFU™ high-fidelity DNA polymerases is supplied with standard 5X HF Buffer, which can be used for complex or GC-rich templates.