Picogreen is an extremely sensitive fluorescent nucleic acid dye, which only emits fluorescence after binding with DNA double strand, and the generated fluorescence is proportional to the DNA concentration, with high sensitivity and strong specificity.

HyperLight^TM PicoGreen dsDNA Assay Kit based on PicoGreen dye is a product designed for fluorescence detection and quantification of double-stranded DNA. It is very sensitive and can withstand higher concentrations of salt, urea, detergent, protein, etc., with convenient operation. It can be used to read samples with high throughput and has a wide range of application. In contrast, the conventional detection method of DNA content is to measure its light absorption value at 260nm (A260), the main disadvantage is that nucleotides, single-stranded nucleic acids and proteins have a great impact on the signal, and will also be interfered by pollutants during the nucleic acid preparation process, and can not distinguish DNA and RNA. PicoGreen quantitative assay kit can be used for cDNA library construction, purification of subclonal DNA fragments, DNA quantification, product amplification and further detection of primers.