HXK1 Rabbit Polyclonal Antibody

Cat# R1093HRPS

Size : 100ug

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Specifications
Product Data
Application ELISA, WB
Application Western blot: 1/500-1/1,000.
ELISA: 1/1,000-1/2,500.
This product has been assayed against 1.0 µg of Hexokinase [Yeast] in a standard capture ELISA using peroxidase conjugated strepavidin and ABTS as a substrate for 30 minutes at room temperature.
Reactivity Yeast
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Hexokinase from Yeast
Specificity This antibody detects Hexokinase [Yeast]. Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined.
Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified Hexokinase [Yeast].
Buffer 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: HRP
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate
Label: Horseradish peroxidase
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration lot specific
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation HRP
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Database Link
Synonyms HXK1, HKA, Hexokinase PI, Hexokinase-A
Reference Data
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