Human IgM (Mu chain specific) goat polyclonal antibody, TRITC
Specifications
Product Data
Recommended Dilution
Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivity
Human
Antibody Host
Goat
Clonality
Polyclonal
Immunogen
Human IgM mu heavy chain.
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: TRITC State: Lyophilized purified Ig fraction. Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 3.1 moles TRITC per mole of Goat IgG.
Reconstitution Method
Restore with 1.0 ml of deionized water (or equivalent).
Concentration
lot specific
Purification
Immunoaffinity chromatography.
Conjugation
TRITC
Storage
Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
