Hepatic Co-Culture Model Cryopreserved Kit, Human/Human

Human/Human Hepatic Co-Culture Model Cryopreserved Kit||Cryopreserved adult and neonatal hepatocytes require special handling to ensure optimal viability following the freeze/thaw process. Please follow these methods closely to ensure the best performance of human hepatocytes in culture.||Kit Components:|1x vial Cryopreserved Human Hepatocytes|1x vial Cryopreserved Growth Arrested Human Liver Stromal Cells. |1x Bottle of Cryopreserved Hepatocyte Recovery Media|1x Bottle of Hepatocyte Plating Media|1x Bottle of Hepatocyte Culture Media ||Required Equipment:|1. All procedures should be conducted inside a biological safety cabinet such as a NuAire Class II Type A2.|2. Incubator should be controlled for humidity, temperature and CO2 concentration such as a NuAire 4950 with the following settings|a. 6% CO2|b. 95% humidity|c. 37°C|Note: It is best to fyrite incubators regularly to ensure appropriate gas concentration is maintained. ||Required Supplies:|1. Fresh or cryopreserved Human hepatocytes|2. Cryopreserved growth arrested Human liver stromal cells|3. Cryopreserved Hepatocyte Recovery Media|4. Hepatocyte Plating Media|5. Hepatocyte Culture Medium|6. Collagen I coated plates |7. Liver biomatrix coated plates||Storage and Stability: |Upon receipt of the cells, immediately transfer the vials into your liquid nitrogen freezer.