Green Fluorescent Protein (GFP)

Green fluorescent protein (GFP), a 27kD protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelenth of 509nm) when excited by blue light (excitation peak at a wavelenth of 395nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein-protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP is used to measure single cell metastasis and successful proliferation of stem cells. In these ways, GFP technology has contributed to a greater understanding of cellular biology and biochemistry.||Applications:|Suitable for use in ELISA, Immunocytochemistry and Western Blot. Other applications have not been tested.||Recommended Dilution:|ELISA: 1:2000-1:10,000|Western Blot: 1:1000-1:5000. Detects a band of ~28kD. using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods.|Immunocytochemistry: 1:100-1:250.|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.