Goat Anti-Porcine IgG(H+L)-BIOT

Cat# 6050-08

Size : 1.0mg

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Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the heavy and light chains of porcine IgG
Source Pooled antisera from goats hyperimmunized with porcine IgG
Cross Adsorption None; may react with immunoglobulins from other species and the light chains of other porcine immunoglobulins
Purification Method Affinity chromatography on porcine IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-11
FLISA

Other referenced applications for relevant formats include -
Flow Cytometry 1,12
Immunohistochemistry-Paraffin Sections 13
Immunocytochemistry 14
Western Blot 6,7,9,15-24
RRID Number AB_2796124
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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  1. 1. Takamatsu H, Andersen JK, Denyer MS, Parkhouse RM. Establishment of long-term CD154-dependent porcine B-cell cultures. Immunology. 1999;97:211-8. (ELISA, FC)
  2. 2. Hampson DJ, Robertson ID, La T, Oxberry SL, Pethick DW. Influences of diet and vaccination on colonisation of pigs by the intestinal spirochaete Brachyspira (Serpulina) pilosicoli. Vet Microbiol. 2000;73:75-84. (ELISA)
  3. 3. La T, Phillips ND, Reichel MP, Hampson DJ. Protection of pigs from swine dysentery by vaccination with recombinant BmpB, a 29.7 kDa outer-membrane lipoprotein of Brachyspira hyodysenteriae. Vet Microbiol. 2004;102:97-109. (ELISA)
  4. 4. Linghua Z, Xingshan T, Fengzhen Z. The efficacy of CpG oligodinucleotides, in combination with conventional adjuvants, as immunological adjuvants to swine streptococcic septicemia vaccine in piglets in vivo. Int Immunopharmacol. 2006;6:1267-76. (ELISA)
  5. 5. Linghua Z, Yong G, Xingshan T, Fengzhen Z. CpG oligodinucleotides induce strong humoral and cellular responses to swine streptococcic septicemia vaccine in piglets in vivo. Int Immunopharmacol. 2006;6:342-50. (ELISA)
  6. 6. Yao Q, Qian P, Huang Q, Cao Y, Chen H. Comparison of immune responses to different foot-and-mouth disease genetically engineered vaccines in guinea pigs. J Virol Methods. 2008;147:143-50. (ELISA, WB)
  7. 7. Zhou M, Guo Y, Zhao J, Hu Q, Hu Y, Zhang A, et al. Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5. Vaccine. 2009;27:5271-7. (ELISA, WB)
  8. 8. La T, Phillips ND, Hampson DJ. Evaluation of recombinant Bhlp29.7 as an ELISA antigen for detecting pig herds with swine dysentery. Vet Microbiol. 2009;133:98-104. (ELISA)
  9. 9. Li J, Xia J, Tan C, Zhou Y, Wang Y, Zheng C, et al. Evaluation of the immunogenicity and the protective efficacy of a novel identified immunogenic protein, SsPepO, of Streptococcus suis serotype 2. Vaccine. 2011;29:6514-9. (ELISA, WB)
  10. 10. Fu S, Zhang M, Xu J, Ou J, Wang Y, Liu H, et al. Immunogenicity and protective efficacy of recombinant Haemophilus parasuis SH0165 putative outer membrane proteins. Vaccine. 2013;31:347-53. (ELISA)
  11. 11. Hudson LC, Seabolt BS, Odle J, Bost KL, Stahl CH, Piller KJ. Sublethal staphylococcal enterotoxin B challenge model in pigs to evaluate protection following immunization with a soybean-derived vaccine. Clin Vaccine Immunol. 2013;20:24-32. (ELISA)
  12. 12. Grierson SS, King DP, Tucker AW, Donadeu M, Mellencamp MA, Haverson K, et al. Ontogeny of systemic cellular immunity in the neonatal pig: correlation with the development of post-weaning multisystemic wasting syndrome. Vet Immunol Immunopathol. 2007;119:254-68. (FC)
  13. 13. Londoño DP, Alvarez JI, Trujillo J, Jaramillo MM, Restrepo BI. The inflammatory cell infiltrates in porcine cysticercosis: immunohistochemical analysis during various stages of infection. Vet Parasitol. 2002;109:249-59. (IHC-PS)
  14. 14. Tao Q, Fang R, Zhang W, Wang Y, Cheng J, Li Y, et al. Protective immunity induced by a DNA vaccine-encoding Toxoplasma gondii microneme protein 11 against acute toxoplasmosis in BALB/c mice. Parasitol Res. 2013;112:2871-7. (ICC)
  15. 15. Jiang Y, Xiao S, Fang L, Yu X, Song Y, Niu C, et al. DNA vaccines co-expressing GP5 and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV) display enhanced immunogenicity. Vaccine. 2006;24:2869-79. (WB)
  16. 16. Zhang A, Chen B, Mu X, Li R, Zheng P, Zhao Y, et al. Identification and characterization of a novel protective antigen, Enolase of Streptococcus suis serotype 2. Vaccine. 2009;27:1348-53. (WB)
  17. 17. Liao Y, Deng J, Zhang A, Zhou M, Hu Y, Chen H, et al. Immunoproteomic analysis of outer membrane proteins and extracellular proteins of Actinobacillus pleuropneumoniae JL03 serotype 3. BMC Microbiol. 2009;9:172. (WB)
  18. 18. Zhang A, Chen B, Mu X, Zhao Y, Zheng P, Chen H, et al. Identification of three novel in vivo-induced expressed antigens during infection with Streptococcus suis serotype 2. FEMS Microbiol Lett. 2009;295:17-22. (WB)
  19. 19. Zhang A, Mu X, Chen B, Liu C, Han L, Chen H, et al. Identification and characterization of IgA1 protease from Streptococcus suis. Vet Microbiol. 2010;140:171-5. (WB)
  20. 20. Jeong H, Song Y, Lee S, Lee J, Park S, Song C, et al. Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus. Clin Vaccine Immunol. 2010;17:503-12. (WB)
  21. 21. Chen X, Liu J. Generation and immunogenicity of transgenic potato expressing the GP5 protein of porcine reproductive and respiratory syndrome virus. J Virol Methods. 2011;173:153-8. (WB)
  22. 22. Fu Q, Wei Z, Liu X, Xiao P, Lu Z, Chen Y. Glyceraldehyde-3-phosphate dehydrogenase, an immunogenic Streptococcus equi ssp. zooepidemicus adhesion protein and protective antigen. J Microbiol Biotechnol. 2013;23:579-85. (WB)
  23. 23. Zhang R, Li C, He W, Wang C, Xu T, Jin M, et al. Development of latex agglutination test with nucleoprotein as antigen for detection of antibodies to swine influenza virus. Int Immunopharmacol. 2014;19:201-5. (WB)
  24. 24. Lee J, Kwon B, Osorio FA, Pattnaik AK, Lee N, Lee S, et al. Protective humoral immune response induced by an inactivated porcine reproductive and respiratory syndrome virus expressing the hypo-glycosylated glycoprotein 5. Vaccine. 2014;32:3617-22. (WB)

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