Goat anti-Human IgG Fc, HRP conjugated
Cat# AS10791
Size : 1mg
Brand : Agrisera
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Goat anti-Human IgG Fc, HRP conjugated
AS10 791 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG Fc (two heavy chains with constant domains)
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| Immunogen: | Purified human IgG |
| Host: | Goat |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified goat IgG. |
| Format: | Lyophilized |
| Quantity: | 1 mg |
| Reconstitution: | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily |
| Storage: | Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
| Tested applications: | ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) |
| Recommended dilution: | The optimal working dilution should be determined by the investigator |
| Confirmed reactivity: | Human IgG Fc (two heavy chains with constant domains) |
| Predicted reactivity: | Human IgG Fc (two Heavy chains with constant domains) |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
| Additional information: | HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase |
| Additional information (application): | This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis. No reactivity is observed to the light chains on human immunoglobulins or non-immunoglobulin human serum proteins or human IgG F(ab)'2 fragment based on immunoelectrophoresis. |
| Background: | Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays. |