Glutathione-S-Transferase (GST) Activity Assay Kit

Cat# E-BC-K278-S-100

Size : 100Assays

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Detection Principle GST can catalyze the binding of reduced glutathione (GSH) to dinitrobenzene (CDNB) and the product have an absorption peak at 340 nm. The activity of GSH-ST can be calculated by measuring the increasing rate of absorbance at 340 nm.
Synonyms GSH-ST
Sample Type Serum,Plasma,Tissue,Cell
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (340 nm)
Research Area Oxidative Stress ,  Liver And Renal Function
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 1 U/L
Detection Range 1-79 U/L
Precision inter-assay CV: 4.3% | intra-assay CV: 1.9%
Sample Volume 100 μL
Assay Time 50 min
Cat.No. Product Name Sizes
E-BC-K030-M Reduced Glutathione (GSH) Colorimetric Assay Kit 96T , 48T
E-BC-K096-M Glutathione Peroxidase (GSH-Px) Activity Assay Kit 96T , 48T
E-BC-K097-M Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit 96T
E-BC-K099-S Glutathione Reductase (GR) Activity Assay Kit 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum (plasma) 1
10% Rat liver tissue homogenate 150-200
10% Rat lung tissue homogenate 8-12
10% Rat kidney tissue homogenate 10-15
10% Plant tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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