GLR1 Rabbit Polyclonal Antibody
Cat# AP21307BT-N
Size : 1ml
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| Product Data | |
| Application | ELISA, ID, IF, IP, R, WB |
|---|---|
| Application | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques). Working dilutions in non-precipitating antibody-binding techniques: 1/1,000-1/8,000. |
| Reactivity | Bakers Yeast |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Glutathione Reductase isolated and purified from Baker's Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | The antibody recognizes Glutathione Reductase from Baker’s yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
| Buffer | PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized hyperimmune IgG fraction Molar radio: Biotin/IgG ~6.2 |
| Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
| Concentration | lot specific |
| Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
| Conjugation | Biotin |
| Storage | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
| Stability | Shelf life: one year from despatch. |
| Database Link | |
| Background | Glutathione reductase (GR) is a member of pyridine nucleotide-disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione(GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. |
| Synonyms | GRase, GR, GSR, GLUR, GRD1 |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.