ASR2390 | F(ab')2 Anti-Human IgM Fc5u (Peroxidase Conjugated) Secondary

Product Information
Description	F(ab')2 Anti-HUMAN IgM Fc5 µ (GOAT) Antibody Peroxidase Conjugated
Host	Goat
Conjugate	Peroxidase (Horseradish)
Target Species	Human
Clonality	Polyclonal
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, E, IC
Application Note	ELISA 1:20,000 Western Blot 1:2,000-1:10,000 Immunochemistry 1:1,000-1:5,000
Physical State	Lyophilized
Host Isotype	IgG F(ab')2
Target Isotype	IgM Fc5 µ
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen	Human IgM Fc5 µ fragment
Reconstitution Volume	500 µL
Reconstitution Buffer	Restore with deionized water (or equivalent)
Stabilizer	10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	0.01% (w/v) Gentamicin Sulfate

Additional Information
Shipping Condition	Ambient
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG or Human IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

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