ExoBrite™ R-PE IgG1 Isotype Control Flow Antibody (100 tests)
Cat# P008-RPE-500
Size : 500UL
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
| Validated in protein array | Monospecific |
|---|---|
| Antibody number | #P008 |
| Antibody type | Isotype Control |
| Clonality | Monoclonal |
| Host species | Mouse |
| Isotype | IgG1, kappa |
| Antibody reactivity (target) | Isotype Control |
| Shipping condition | Room temperature |
| Antibody application notes | Recommended concentration for flow cytometry: 5 uL per 0.1 mL exosomes |
| Antibody/conjugate formulation | Proprietary buffer containing 0.05% sodium azide |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light |
| Regulatory status | For research use only (RUO) |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
Product Description
ExoBrite™ IgG1 Isotype Control Flow Antibody has been found to not react with any target in human cells and have the same isotype as the ExoBrite™ Flow Antibodies against tetraspanin proteins CD9, CD63, and CD81. The antibody is available with the same conjugates as ExoBrite™ Flow Antibodies for the Pacific Blue™, FITC, PE, and APC channels.
- Non-reactive IgG1 mouse antibody
- Serves as a isotype negative control for ExoBrite™ Flow Antibodies
- Available in 5 colors
The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of EVs, but their expression levels vary depending on the cell type of origin.
EV antibodies you can trust
Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs and may require tedious optimization for your EV prep and staining protocol. The antibodies and dye labels of ExoBrite™ Flow Antibody Conjugates were carefully selected and validated for robust detection of isolated EVs. In addition, the antibodies are provided in a proprietary buffer formulation for reduced antibody aggregation and brighter EV staining for optimal accuracy and signal-to-noise.
For general EV staining, Biotium’s
ExoBrite™ True EV Membrane Stains
offer unparalleled coverage of EVs in a sample and address issues of dye aggregation often seen with PKH and other common membrane dyes. Biotium also offers optimized ExoBrite™ EV Surface stains conjugated tocholera toxin B (CTB)
,wheat germ agglutinin (WGA)
, andAnnexin V
. These stains are specially formulated for bright and specific detection of isolated EVs by flow cytometry. These ExoBrite™ EV Surface stains may also be combined with antibody staining, for multi-parameter analysis.Biotium also offers conjugated ExoBrite™ Western Antibodies against
CD9
,CD63
, andCD81
designed for optimal detection in EV extracts by fluorescent western blot.Note: In our testing, we have found that ExoBrite™ 490/515 dye may bind to streptavidin coated surfaces or beads if free biotin binding sites are not blocked. We recommend performing a biotin blocking step after binding your biotinylated capture antibody to streptavidin beads or surfaces when using ExoBrite™ 490/515 conjugates. Alternatively, consider using a different ExoBrite™ dye for staining EVs captured on streptavidin beads or surfaces.
ExoBrite™ Flow Antibody Conjugates
| Antibody | Ex/Em | Detection Channel | Size | Catalog No. |
|---|---|---|---|---|
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P003-410-125 |
| 100 tests | P003-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P003-490-125 |
| 100 tests | P003-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P003-560-125 |
| 100 tests | P003-560-500 | |||
ExoBrite™ 640/660 | 642/663 nm | APC | 25 tests | P003-640-125 |
| 100 tests | P003-640-500 | |||
ExoBrite™ 650/665 | 652/668 nm | APC | 25 tests | P003-650-125 |
| 100 tests | P003-650-500 | |||
ExoBrite™ R-PE | 496, 546, 565/578 nm | PE | 25 tests | P003-RPE-125 |
| 100 tests | P003-RPE-500 | |||
ExoBrite™ APC | 651/660 nm | APC | 25 tests | P003-APC-125 |
| 100 tests | P003-APC-500 | |||
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P018-410-125 |
| 100 tests | P018-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P018-490-125 |
| 100 tests | P018-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P018-560-125 |
| 100 tests | P018-560-500 | |||
ExoBrite™ 640/660 | 642/663 nm | APC | 25 tests | P018-640-125 |
| 100 tests | P018-640-500 | |||
ExoBrite™ 650/665 | 652/668 nm | APC | 25 tests | P018-650-125 |
| 100 tests | P018-650-500 | |||
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P004-410-125 |
| 100 tests | P004-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P004-490-125 |
| 100 tests | P004-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P004-560-125 |
| 100 tests | P004-560-500 | |||
ExoBrite™ 640/660 | 642/663 nm | APC | 25 tests | P004-640-125 |
| 100 tests | P004-640-500 | |||
ExoBrite™ 650/665 | 652/668 nm | APC | 25 tests | P004-650-125 |
| 100 tests | P004-650-500 | |||
ExoBrite™ R-PE | 496, 546, 565/578 | PE | 25 tests | P004-RPE-125 |
| 100 tests | P004-RPE-500 | |||
ExoBrite™ APC | 651/660 nm | APC | 25 tests | P004-APC-125 |
| 100 tests | P004-APC-500 | |||
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P022-410-125 |
| 100 tests | P022-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P022-490-125 |
| 100 tests | P022-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P022-560-125 |
| 100 tests | P022-560-500 | |||
ExoBrite™ 640/660 | 642/663 nm | APC | 25 tests | P022-640-125 |
| 100 tests | P022-640-500 | |||
ExoBrite™ 650/665 | 652/668 nm | APC | 25 tests | P022-650-125 |
| 100 tests | P022-650-500 | |||
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P005-410-125 |
| 100 tests | P005-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P005-490-125 |
| 100 tests | P005-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P005-560-125 |
| 100 tests | P005-560-500 | |||
ExoBrite™ 640/660 | 642/663 nm | APC | 25 tests | P005-640-125 |
| 100 tests | P005-640-500 | |||
ExoBrite™ 650/665 | 652/668 nm | APC | 25 tests | P005-650-125 |
| 100 tests | P005-650-500 | |||
ExoBrite™ R-PE | 496, 546, 565/578 | PE | 25 tests | P005-RPE-125 |
| 100 tests | P005-RPE-500 | |||
ExoBrite™ APC | 651/660 nm | APC | 25 tests | P005-APC-125 |
| 100 tests | P005-APC-500 | |||
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P019-410-125 |
| 100 tests | P019-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P019-490-125 |
| 100 tests | P019-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P019-560-125 |
| 100 tests | P019-560-500 | |||
ExoBrite™ 640/660 | 642/663 nm | APC | 25 tests | P019-640-125 |
| 100 tests | P019-640-500 | |||
ExoBrite™ 650/665 | 652/668 nm | APC | 25 tests | P019-650-125 |
| 100 tests | P019-650-500 | |||
ExoBrite™ 410/450 IgG1 | 416/452 nm | Pacific Blue ™ | 25 tests | P008-410-125 |
| 100 tests | P008-410-500 | |||
ExoBrite™ 490/515 IgG1 | 490/516 nm | FITC | 25 tests | P008-490-125 |
| 100 tests | P008-490-500 | |||
ExoBrite™ 560/585 IgG1 | 562/584 nm | PE | 25 tests | P008-560-125 |
| 100 tests | P008-560-500 | |||
ExoBrite™ R-PE IgG1 | 496, 546, 565/578 | PE | 25 tests | P008-RPE-125 |
| 100 tests | P008-RPE-500 | |||
| ExoBrite™ 640/660 IgG1 | 642/663 nm | APC | 25 tests | P008-640-125 |
| 100 tests | P008-640-500 | |||
ExoBrite™ 650/665 IgG1 | 652/668 nm | APC | 25 tests | P008-650-125 |
| 100 tests | P008-650-500 |