CytoLiner™ Fixed Cell Membrane Dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.

Novel Membrane Dyes Designed for Fixed Cells

While classic lipophilic membrane dyes like DiI can be used to stain formaldehyde-fixed cells, staining can be highly variable due to the poor solubility of the dyes. CytoLiner™ Dyes are a new generation of membrane dyes uniquely engineered to permit selective staining of the plasma membrane in fixed and mildly permeabilized cells. CytoLiner™ staining is compatible with formaldehyde fixation and mild detergent permeabilization before staining. Staining can tolerate blocking agents used during immunofluorescence staining protocols, allowing subsequent staining with antibodies and other probes. The dyes also are compatible with poly-L-lysine coated cultureware and Transwell® permeable filter supports. CytoLiner™ Dyes are available in a selection of 5 colors from blue to near-IR.

Superior Staining Performance Over Classic Membrane Dyes

Suitable for Co-Staining with Antibodies and Other Probes

Find the Right Stain for Your Application

CytoLiner™ Dyes are part of Biotium’s family of unique and innovative cell membrane stains designed for specific applications. For workflows requiring staining live cells before fixation, we recommend our

CellBrite® Fix Membrane Stains

 or 

MemBrite® Fix Cell Surface Staining Kits

. Biotium also offers CF® Dye conjugated lectins, including

Concanavalin A (Con A)

and

Wheat Germ Agglutinin (WGA)

, for visualizing cell surfaces in live or fixed cells.  For longer term monitoring of cell surfaces in live cells, we recommend

CellBrite® Steady Dyes

. To find the right stain for your application, see our

Membrane & Cell Surface Stains Comparison

, or download our 

Membrane & Surface Stains Brochure

.

CytoLiner™ Fixed Cell Membrane Stains

Product	Ex/Em	Detection Channels	Size	Catalog Number
CytoLiner™ 410/450	406/446 nm	DAPI/Pacific Blue™	250 Labelings	30131-T
			1000 Labelings	30131
CytoLiner™ 495/510	492/510 nm	FITC	250 Labelings	30132-T
			1000 Labelings	30132
CytoLiner™ 570/590	573/592 nm	Cy®3/TRITC	250 Labelings	30133-T
			1000 Labelings	30133
CytoLiner™ 650/675	647/674 nm	Cy®5	250 Labelings	30134-T
			1000 Labelings	30134
CytoLiner™ 680/710	682/707 nm	Cy®5.5	250 Labelings	30135-T
			1000 Labelings	30135