Creatinine (Cr) BioAssay™ Kit (Colorimetric)

Creatinine is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for highenergy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and pcreatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from pcreatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is <15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.||For quantitative detection of creatinine in serum, plasma and urine.||Detection Range:|25-800umol/L||Sensitivity: |10umol/L||Precision: |Intra-Assay %CV: <6% |Inter-Assay %CV: <4% ||Specificity:|This assay has high sensitivity and excellent specificity for detection of creatinine. No significant crossreactivity or interference between creatinine and analogues was observed.||Kit Components:|Microtiter Strips, 1x96 wells|Lyophilized Standard(1000nmol/vial), 2x vial|Enzyme Solution A, 1x25ml|Enzyme Solution B, 1x8ml||Storage and Stability:|Store unopened Microtiter Strips at 4ºC; store at -20ºC once opened. Store unopened Standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.