Coronavirus (COVID-19) Nucleic Acid BioGenomics™ Kit (RT-PCR) (Research Use Only)

COVID-19 (Corona Virus Disease) is an infectious disease caused by the most recently discovered coronavirus. This new virus and disease were unknown before the outbreak began in Wuhan, China, in December 2019. The most common symptoms of COVID-19 are fever, tiredness, and dry cough. Some patients may have aches and pains, nasal congestion, runny nose, sore throat or diarrhea. These symptoms are usually mild and begin gradually. Some people become infected but don't develop any symptoms and don't feel unwell. Most people (about 80%) recover from the disease without needing special treatment. Around 1 out of every 6 people who gets COVID-19 becomes ill seriously and has difficult breathing among senior people, and those with underlying medical problems like high blood pressure, heart problems or diabetes, are more likely to develop serious illness. About 2% of people with the disease have died. People with fever, cough and difficulty breathing should seek medical attention. People can catch COVID-19 from others who carry the virus. The disease can spread from person to person through small droplets from the nose or mouth when a person with COVID-19 coughs or exhales. These droplets land on objects or surfaces around the patients, like soap or towel that patients have used. Other people then may catch COVID-19 by touching those objects or surfaces, then touching their eyes, nose or mouth. People can also catch COVID-19 if they massage the hand without touching the puncture site by rubbing breathe in droplets from a person with COVID-19 who coughs out or exhales droplets. The incubation period for COVID-19 generally ranges from 1-14 days.||Sample Type: |Pharynx swab, nasopharyngeal extract, sputum, bronchial lavage fluid, alveolar lavage fluid, etc||Intended Use:|Coronavirus (COVID-19) Nucleic Acid BiioGenomics™ Kit (RT-PCR) is used for the qualitative detection of nucleic acids of novel coronavirus (2019). The test results can only be used for clinical auxiliary diagnosis and cannot be used as the basis for diagnosis or exclusion of cases alone. The Coronavirus (CoV) belongs to the order Nestoroviridae, family Coronaviridae. This family is divided into four genera: α, β, γ, and δ according to serotype and genomic characteristics. Previously there were six reported coronavirus infections in humans, including 229E and NL63 of the α genus and OC43, MERS-CoV and SARS- CoV of β genus. CoV is mainly transmitted through direct contact with secretions or through aerosols and droplets, and there is evidence that it can be transmitted through the fecaloral dissemination.||Coronavirus (COVID-19) belongs to β genus and has a capsule. The particles are round or oval and often multiform with a diameter of 60-140 nm. There are obvious differences between genetic characteristics of COVID-19, SARS-CoV and MERS-CoV. The present study shows that it has more than 85% homology with SARS like coronavirus originated from bat (bat-SL-CoVZC45).|The clinical symptoms of novel coronavirus pneumonia are fever, fatigue, and other systemic symptoms, accompanied by dry cough, dyspnea, and so on. It can rapidly develop into severe pneumonia, respiratory failure, acute respiratory distress syndrome, septic shock, metabolic acidosis difficult to correct and coagulation dysfunction, and multiple organ failure leading to death. At present, it is mainly being detected by virus isolation, nucleic acid detection, and serological detection. Kit is suitable for RUO (Research Use Only).||Test Principle:|This kit uses Multiplex Fluorescence RT-PCR and hydrolytic probe techniques to design specific primers and probes targeting conserved regions in ORF1ab genes and N genes of COVID-19. The qualitative detection of nucleic acid levels at two target sites is achieved in the same reaction tube. The operation is simple, the whole process does not need open the cover, reducing contamination. The instrument software systems recommended automatically draw the real- time amplification curve to determine the instant test results. This kit is equipped with internal standard, which is used to monitor the collection, transportation and extraction of test samples to avoid false negative test results.||Kit Components (24T/48T):|COVID-19 qPCR Mix, 400ul/800ul|Enzyme Mix, 25ul/50ul|Positive Control, 25ul/50ul |Negative Control, 100ul/200ul ||Storage and Stability:|Kit can be stored at 20°C in the dark, stable for 6 months after receipt. It is recommended to repackage the reagents and freeze them. Avoid repeated freeze-thaw cycles.