CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.
Chemical Properties
Physical Appearance
A solid
Storage
Store at -20°C
M.Wt
614.88
Cas No.
75621-03-3
Formula
C32H58N2O7S
Solubility
≥61.5 mg/mL in H2O; insoluble in EtOH; insoluble in DMSO