CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: Psel.KO.2.12]

Specifications

Product Data
Clone Name	Psel.KO.2.12
Applications	FC, IHC, IP
Recommended Dilution	Immunoprecipitation. Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cell in 100 µl.
Reactivities	Goat, Human, Rat, Sheep
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	CD62P transfected 300.19 cells.  Spleen cells from immunised CD62P knock-out mice (Strain C57/B6) were fused with cells of the NS-1 myeloma cell line.
Specificity	This antibody recognizes the CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. Clone Psel.KO.2.12 is reported to inhibit P-Selectin-dependent adhesion between HL60 cells and P-Selectin transfected COS cells (Ref.1). We recommend the use of SM2262LE for this purpose.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from tissue culture supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	selectin P
Database Link	Entrez Gene 6403 Human UniProt ID P16109
Background	CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
Synonyms	SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3
Reference Data