CD62P (P-Selectin) (PE)

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Cat# 214717-100T

Size : 100Tests

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Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

214717 CD62P (P-Selectin) (PE)

Clone Type

Polyclonal

Host

mouse

Source

human

Swiss Prot

P16109

Isotype

IgG1

Grade

Affinity Purified

Applications

Crossreactivity

Hu Mk

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

CD62P is also known as P-selectin, PADGEM or GMP140 and plays an important role in adhesive processes between leucocytes and endothelial cells. CD62P is a component of the alpha granule and is translocated to the plasma membrane upon activation.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at 4°C. Reconstitute with sterile dH2O. Aliquot and store at 4°C. Reconstituted product is stable for 6 months as an undiluted liquid. Dilute only prior to immediate use. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: AK-6|Host: mouse|Source: human|Concentration: ~1mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder in PBS, 5% sucrose, 1% BSA, 0.09% sodium azide. Reconstitute with 0.25ml sterile dH2O PBS. Labeled with R-Phycoerythrin (PE). |Purity: Purified by Protein G affinity chromatography.|Immunogen: Human platelet membrane glycoproteins.|Specificity: Recognizes human CD62P. Species Crossreactivity: Rhesus monkey ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human platelet membrane glycoproteins.

Form

Supplied as a lyophilized powder in PBS, 5% sucrose, 1% BSA, 0.09% sodium azide. Reconstitute with 0.25ml sterile dH2O PBS. Labeled with R-Phycoerythrin (PE).

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes human CD62P. Species Crossreactivity: Rhesus monkey

References

1. Dunlop, L.C. et al. (1992) Characterization of GMP-140 (P-selectin) as a circulating plasma protein. J. Exp. Med. 175: 1147-1150. 2. Skinner, M.P. et al. (1989) Characterization of human platelet GMP-140 as a heparin-binding protein. Biochem. Biophys. Res. Commun. 164 (3): 1373-1379. 3. Skinner, M.P. et al. (1991) GMP-140 binding to neutrophils is inhibited by sulfated glycans. J. Biol. Chem. 266: 5371-5374. 4. Bevilacqua, M.P. et al. (1993) Selectins J. Clin. Invest. 91: 379. 5. Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of Rhesus monkeys. Cytometry 29: 351-362. 6. Kitaya, K. & Yasuo, T. (2010) Aberrant expression of selectin E, CXCL1, and CXCL13 in chronic endometritis. . PubMed PMID: Mod Pathol. May 21 [Epub ahead of print] 7. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil- derived microparticles. Blood. 112: 2512-2519. 8. Knipe, L. et al (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells. Blood. 116: 2183-91. 9. Kornerup, K.N. et al. (2010) Circulating platelet-neutrophil complexes are important for subsequent neutrophil activation and migration. J Appl Physiol. 109: 758-67. 10. Norling, L.V. et al. (2008) Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking. FASEB J. 22: 682-90. 11. van Nispen tot Pannerden, H. et al. (2010) The platelet interior revisited: electron tomography reveals tubular alpha-granule subtypes. Blood. 116: 1147-56. 12. Wassmer, S.C. at al. (2008) Platelet-induced clumping of Plasmodium falciparum-infected erythrocytes from Malawian patients with cerebral malaria-possible modulation in vivo by thrombocytopenia. J Infect Dis. 197: 72-8. 13. Theoret, J.F. et al. (2001) P-selectin antagonism with recombinant p-selectin glycoprotein ligand-1 (rPSGL-Ig) inhibits circulating activated platelet binding to neutrophils induced by damaged arterial surfaces. J Pharmacol Exp Ther. 298: 658-64 14. Turner, C.P. et al. (2003) The role of P-selectin in the immune destruction of platelets. Br J Haematol. 121: 623-31.