OKCD04366 | CA125 ELISA Kit (Mouse) Biotrend

CA125 ELISA Kit (Mouse) (OKCD04366)

Datasheets/Manuals	Printable datasheet for CA125 ELISA Kit (Mouse) (OKCD04366)

Product Info

Predicted Species Reactivity

Mouse

Application

ELISA-Sandwich

ELISA Kit Detection Method

Colorimetric

ELISA Kit Duration

ELISA Kit Linearity

Sample	1:2	1:4	1:8	1:16
Serum (n=5)	87-101%	80-89%	95-103%	98-105%
EDTA Plasma (n=5)	97-105%	92-101%	98-105%	78-104%
Heparin Plasma (n=5)	91-103%	80-101%	91-98%	80-93%

ELISA Kit Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Antigen 125 (CA125). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbohydrate Antigen 125 (CA125). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbohydrate Antigen 125 (CA125), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbohydrate Antigen 125 (CA125) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ELISA Kit Range

0.156-10ng/mL

ELISA Kit Recovery

Matrix	Recovery range (%)	Average (%)
Serum (n=5)	80-91	86
EDTA Plasma (n=5)	92-105	101
Heparin Plasma (n=5)	97-105	101

ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbohydrate Antigen 125 (CA125) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbohydrate Antigen 125 (CA125) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

ELISA Kit Component

Component	Amount
Ca125 Microplate	96 Wells (12 x 8 Well strips)
Ca125 Lyophilized Standard	2
100X Ca125 HRP-Detector Anitbody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Standard Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
30X Wash Buffer	1 x 20 mL
100X Luminol Substrate	1 x 2 mL
Substrate Diluent	1 x 20 mL

Reconstitution and Storage

2°C to 8°C|-20°C

Sample Type

Serum, plasma, tissue homogenates and other biological fluids

Sensitivity

6.5 pg/mL

Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Target Info

Gene Symbol	CA125
Protein Name	Carbohydrate Antigen 125
Uniprot ID	Q9D1H1

Protocols & Data

CA125 Antibody (OACD02106)
Catalog #: OACD02106

Application: ICC|IHC|IHC-P|IP|WB

Format: Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

CA125 ELISA Kit (Mouse)

Cat# OKCD04366

Contact local distributor :

Brand : Aviva Systems Biology

Contact local distributor :

CA125 ELISA Kit (Mouse) (OKCD04366)

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