BOVINE SERUM ALBUMIN 30% Solution
Cat# BSA-30
Size : 500mL
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for Bovine Serum Albumin 30% Solution
Product Details
Bovine Serum Albumin 30% Solution - BSA-30
BOVINE SERUM ALBUMIN, BSA, BSA Blocker, BSA Blocking, BSA30
Bovine
Target Details
BOVINE SERUM ALBUMIN 30% Solution contains a 30% solution of bovine serum albumin (Fraction V, Immunoglobulin and Protease Free) in saline with preservative and stabilizer. The product is ~30% protein as determined by Biuret assay. A clear yellow green to dark amber color is typical. This product is prepared from healthy adult animals and is processed from pooled lots of serum to ensure uniformity.
Application Details
Biochemical Assay, ELISA, Other - View References
BOVINE SERUM ALBUMIN 30% Solution is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody, and use as a blocking agent to reduce non-specific binding.
Formulation
30% by dry weight
0.85% (w/v) Sodium Chloride
0.01% (w/v) Sodium Azide
0.3% (v/v) Octanoic Acid
Shipping & Handling
Wet Ice
Store Blocking Buffer at 4° C prior to opening. DO NOT FREEZE.
Expiration date is six (6) months from date of receipt.
Background
Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Sterile validated preparations may be used as a nutrient in cell and microbial culture. In restriction digests, specially prepared BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
References (6)
Yang X et al. (2022). Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples. STAR Protoc.
Applications
EIA, ELISA PubMed
Christensen SC et al. (2020). Characterization of basigin monoclonal antibodies for receptor-mediated drug delivery to the brain. Sci Rep.
Applications
Kinase Assay PubMed
Ferdman J et al. (2019). Intra-seasonal antibody repertoire analysis of a subject immunized with an MF59®-adjuvanted pandemic 2009 H1N1 vaccine. Vaccine.
Applications
E, EIA PubMed
Raymond DD et al. (2016). Influenza immunization elicits antibodies specific for an egg-adapted vaccine strain. Nat Med.
Applications
NEU PubMed
Schmidt AG et al. (2015). Viral receptor-binding site antibodies with diverse germline origins. Cell.
Applications
NEU PubMed
Keeler, GD et al. (2014). Comparison of microdialysis sampling perfusion fluid components on the foreign body reaction in rat subcutaneous tissue. European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
Applications
Buffer PubMed
Related Protocols
Adherent Cell Lysis Protocol
Biotin Binding Assay Protocol
ELISA Protocol
Nuclear & Cytoplasmic Extract Protocol
Peptide Competition Assay (PCA) Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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