Birch pollen allergen recombinant monoclonal antibody, clone BV16

Catalog # RAB03700

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Price

Stock

Quantity

Size:200 ug
Price: USD $ 850.00
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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Birch pollen allergen recombinant monoclonal antibody, clone BV16 (Cat # RAB03100).
Binding curve of RAB03100 to ELISA Plate coated with BETVIA at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using RAB03100. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used. e at 450nm does not reach a plateau but falls slightly before increasing once more- as also observed for 3.0. This is suggestive of two binding sites on Bet v 1-A for which RAB03100 has differing affinities.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against Birch pollen allergen (BETVIA).Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against Birch pollen allergen, a major allergen of the European white birch.

    Reactivity

    Plants

    Form

    Liquid

    Isotype

    IgG kappa

    Recommend Usage

    Crystallography
    ELISA
    Western Blot
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS with 0.02% Proclin 300

    Storage Instruction

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Birch pollen allergen recombinant monoclonal antibody, clone BV16 (Cat # RAB03100).
    Binding curve of RAB03100 to ELISA Plate coated with BETVIA at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using RAB03100. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used. e at 450nm does not reach a plateau but falls slightly before increasing once more- as also observed for 3.0. This is suggestive of two binding sites on Bet v 1-A for which RAB03100 has differing affinities.

    Crystallography