Biotin-PEG4-azide [1309649-57-7]
Cat# HY-140910-100mg
Size : 100mg
Brand : MedChemExpress
Biotin-PEG4-azide is a biotin-labeled, PEG-based PROTAC linker that can be used in the synthesis of PROTACs. Biotin-PEG4-azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
Biotin-PEG4-azide Chemical Structure
CAS No. : 1309649-57-7
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Description |
Biotin-PEG4-azide is a biotin-labeled, PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1]. Biotin-PEG4-azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups. |
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IC50 & Target |
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In Vitro |
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
488.60 |
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Formula |
C20H36N6O6S |
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CAS No. |
1309649-57-7 |
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Appearance |
Solid |
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Color |
White to yellow |
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SMILES |
O=C(NCCOCCOCCOCCOCCN=[N+]=[N-])CCCC[C@@H]1SC[C@]([C@]1(22)N2)(22)NC2=O |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
-20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
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Solvent & Solubility |
In Vitro:
DMSO : ≥ 100 mg/mL (204.67 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation | |||||||||||||
References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.0467 mL | 10.2333 mL | 20.4666 mL | 51.1666 mL |
5 mM | 0.4093 mL | 2.0467 mL | 4.0933 mL | 10.2333 mL | |
10 mM | 0.2047 mL | 1.0233 mL | 2.0467 mL | 5.1167 mL | |
15 mM | 0.1364 mL | 0.6822 mL | 1.3644 mL | 3.4111 mL | |
20 mM | 0.1023 mL | 0.5117 mL | 1.0233 mL | 2.5583 mL | |
25 mM | 0.0819 mL | 0.4093 mL | 0.8187 mL | 2.0467 mL | |
30 mM | 0.0682 mL | 0.3411 mL | 0.6822 mL | 1.7056 mL | |
40 mM | 0.0512 mL | 0.2558 mL | 0.5117 mL | 1.2792 mL | |
50 mM | 0.0409 mL | 0.2047 mL | 0.4093 mL | 1.0233 mL | |
60 mM | 0.0341 mL | 0.1706 mL | 0.3411 mL | 0.8528 mL | |
80 mM | 0.0256 mL | 0.1279 mL | 0.2558 mL | 0.6396 mL | |
100 mM | 0.0205 mL | 0.1023 mL | 0.2047 mL | 0.5117 mL |
Biotin-PEG4-azide Related Classifications
- PROTAC
- PROTAC Linkers
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
Biotin-PEG4-azide1309649-57-7PROTAC LinkersInhibitorinhibitorinhibit