Detection of human IgG1 in monkey serum, using
RM117 (capture) and biotin-RM129 (detection)
as a Sandwich ELISA pair. HRP conjugated
streptavidin and TMB were used to yield the
colorimetric reaction.

ELISA showing RM129 reacts only to kappa and
lambda light chain of all human immunoglobulins,
not to mouse IgG, rat IgG, or goat IgG.

ELISA showing RM129 does not react to monkey
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM129 and a monkey IgG binding
antibody (positive control) was used as the detection
antibody.

A titer Sandwich ELISA using Biotinylated RM129
as the detection antibody. The plate, coated
with the capture antibody anti-human IgG RM116,
was loaded with different amounts of human IgG.
A serial dilution of Biotin RM129 was used as
the detection antibody, followed by an alkaline
phosphatase conjugated streptavidin.