Atg101 (NM_026566) Mouse Tagged ORF Clone

CAT#: MR202440

  • TrueORF®

9430023L20Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9430023L20 gene (9430023L20Rik)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK

Datasheet
SDS
Plasmid Map

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Product Images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Atg101
Synonyms 9430023L20Rik; AA407441; AU022967
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR202440 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTGTCGATCAGAAGTGCTGGAGGTGTCGGTGGAGGGGCGGCAGGTGGAGGAGGCCATGCTGGCGG
TGCTGCACACGGTGCTCCTGCACCGCAGCACGGGCAAGTTTCACTACAAGAAGGAGGGCACGTACTCCAT
AGGCACCGTGGGCATCCAGGATGTAGACTGTGACTTCATCGACTTCACCTATGTGCGCGTCTCCTCAGAG
GAGCTGGACCGTGCCTTGCGCAAGGTTGTTGGGGAATTCAAGGATGCACTGAGGAACTCAGGTGGTGATG
GCCTGGGCCAGATGTCCTTGGAATTCTATCAGAAGAAAAAGTCTCGATGGCCTTTTTCGGATGAGTGCAT
CCCCTGGGAGGTGTGGACTGTCAAGGTGCACGTGGTAGCCTTGGCCACAGAGCAGGAGCGGCAGATATGC
AGGGAGAAGGTGGGCGAGAAGCTGTGCGAGAAGATCATTAACATCGTGGAGGTGATGAGCCGGCATGAGT
ACCTGCCCAAGATGCCCACGCAGTCAGAGGTGGACAACGTGTTTGACACGGGCCTGCGGGACGTGCAGCC
CTACCTCTACAAGATCTCCTTTCAGATCACCGAGGCCCTGGGCACCTCAGTCACCACCACCATGCGCAGG
CTCATCAAAGACACCCTTGCCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR202440 protein sequence
Red=Cloning site Green=Tags(s)

MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGIQDVDCDFIDFTYVRVSSE
ELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWEVWTVKVHVVALATEQERQIC
REKVGEKLCEKIINIVEVMSRHEYLPKMPTQSEVDNVFDTGLRDVQPYLYKISFQITEALGTSVTTTMRR
LIKDTLAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_026566
ORF Size 657 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_026566.2, NP_080842.1
RefSeq Size 1230 bp
RefSeq ORF 657 bp
Locus ID 68118
UniProt ID Q9D8Z6
Cytogenetics 15 F1
MW 25 kDa
Gene Summary Autophagy factor required for autophagosome formation. Stabilizes ATG13, protecting it from proteasomal degradation.[UniProtKB/Swiss-Prot Function]

Other Versions

SKU Description Size
MC210694 9430023L20Rik (untagged) - Mouse RIKEN cDNA 9430023L20 gene (9430023L20Rik), (10ug)
    • 10 ug

330,00 EUR

MG202440 9430023L20Rik (tGFP-tagged) - Mouse RIKEN cDNA 9430023L20 gene (9430023L20Rik)
    • 10 ug

500,00 EUR

MR202440L3 Lenti ORF clone of 9430023L20Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9430023L20 gene (9430023L20Rik)
    • 10 ug

600,00 EUR

MR202440L4 Lenti ORF clone of 9430023L20Rik (mGFP-tagged) - Mouse RIKEN cDNA 9430023L20 gene (9430023L20Rik)
    • 10 ug

600,00 EUR