Atg101 (NM_026566) Mouse Tagged ORF Clone
CAT#: MR202440
- TrueORF®
9430023L20Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9430023L20 gene (9430023L20Rik)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Atg101 |
Synonyms | 9430023L20Rik; AA407441; AU022967 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data | >MR202440 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTGTCGATCAGAAGTGCTGGAGGTGTCGGTGGAGGGGCGGCAGGTGGAGGAGGCCATGCTGGCGG TGCTGCACACGGTGCTCCTGCACCGCAGCACGGGCAAGTTTCACTACAAGAAGGAGGGCACGTACTCCAT AGGCACCGTGGGCATCCAGGATGTAGACTGTGACTTCATCGACTTCACCTATGTGCGCGTCTCCTCAGAG GAGCTGGACCGTGCCTTGCGCAAGGTTGTTGGGGAATTCAAGGATGCACTGAGGAACTCAGGTGGTGATG GCCTGGGCCAGATGTCCTTGGAATTCTATCAGAAGAAAAAGTCTCGATGGCCTTTTTCGGATGAGTGCAT CCCCTGGGAGGTGTGGACTGTCAAGGTGCACGTGGTAGCCTTGGCCACAGAGCAGGAGCGGCAGATATGC AGGGAGAAGGTGGGCGAGAAGCTGTGCGAGAAGATCATTAACATCGTGGAGGTGATGAGCCGGCATGAGT ACCTGCCCAAGATGCCCACGCAGTCAGAGGTGGACAACGTGTTTGACACGGGCCTGCGGGACGTGCAGCC CTACCTCTACAAGATCTCCTTTCAGATCACCGAGGCCCTGGGCACCTCAGTCACCACCACCATGCGCAGG CTCATCAAAGACACCCTTGCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202440 protein sequence Red=Cloning site Green=Tags(s) MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGIQDVDCDFIDFTYVRVSSE ELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWEVWTVKVHVVALATEQERQIC REKVGEKLCEKIINIVEVMSRHEYLPKMPTQSEVDNVFDTGLRDVQPYLYKISFQITEALGTSVTTTMRR LIKDTLAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_026566 |
ORF Size | 657 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026566.2, NP_080842.1 |
RefSeq Size | 1230 bp |
RefSeq ORF | 657 bp |
Locus ID | 68118 |
UniProt ID | Q9D8Z6 |
Cytogenetics | 15 F1 |
MW | 25 kDa |
Gene Summary | Autophagy factor required for autophagosome formation. Stabilizes ATG13, protecting it from proteasomal degradation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | |
---|---|---|---|
MC210694 | 9430023L20Rik (untagged) - Mouse RIKEN cDNA 9430023L20 gene (9430023L20Rik), (10ug) | 330,00 EUR | |
MG202440 | 9430023L20Rik (tGFP-tagged) - Mouse RIKEN cDNA 9430023L20 gene (9430023L20Rik) | 500,00 EUR | |
MR202440L3 | Lenti ORF clone of 9430023L20Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9430023L20 gene (9430023L20Rik) | 600,00 EUR | |
MR202440L4 | Lenti ORF clone of 9430023L20Rik (mGFP-tagged) - Mouse RIKEN cDNA 9430023L20 gene (9430023L20Rik) | 600,00 EUR |