Anti-TRIM27 antibody (1-200)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	IHC-P 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	TRIM27
Gene ID:	5987
Uniprot ID:	TRI27_HUMAN
Immunogen Region:	1-200
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TRIM27 (NP_006501.1).
Immunogen Sequence:	MASGSVAECLQQETTCPVCL QYFAEPMMLDCGHNICCACL ARCWGTAETNVSCPQCRETF PQRHMRPNRHLANVTQLVKQ LRTERPSGPGGEMGVCEKHR EPLKLYCEEDQMPICVVCDR SREHRGHSVLPLEEAVEGFK EQIQNQLDHLKRVKDLKKRR RAQGEQARAELLSLTQMERE KIVWEFEQLYHSLKEHEYRL

Tissue Specificity	Expressed in testis namely within the seminiferous tubules.
Function	E3 ubiquitin-protein ligase that mediates ubiquitination of various substrates and thereby plays a role in diffent processes including proliferation, innate immunity, apoptosis, immune response or autophagy. Ubiquitinates PIK3C2B and inhibits its activity by mediating the formation of 'Lys-48'-linked polyubiquitin chains.the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA. Forms a complex with and ubiquitinates the ubiquitin-specific protease USP7, which in turn deubiquitinates RIPK1 resulting in the positive regulation of TNF-alpha-induced apoptosis. In addition, acts with USP7 or PTPN11 as an inhibitor of the antiviral signaling pathway by promoting kinase TBK1 ubiquitination and degradation. Acts as a negative regulator of NOD2 signaling by mediating ubiquitination of NOD2, promoting its degradation by the proteasome. Alternatively, facilitates mitophagy via stabilization of active TBK1. Negatively regulates autophagy flux under basal conditions by directly polyubiquitinating ULK1. During starvation-induced autophagy, catalyzes non-degradative ubiquitination of the kinase STK38L promoting its activation and phosphorylation of ULK1 leading to its ubiquitination and degradation to restrain the amplitude and duration of autophagy. (Microbial infection) Positively regulates hepatitis C virus replication by suppressing type I IFN response during infection.
Protein Name	Zinc Finger Protein Rfp Ring Finger Protein 76 Ret Finger Protein Tripartite Motif-Containing Protein 27
Database Links	Reactome: R-HSA-3232142 Reactome: R-HSA-8948751 Reactome: R-HSA-9635465
Cellular Localisation	Nucleus Cytoplasm Pml Body Early Endosome Mitochondrion Nuclear Or Cytoplasmic Depending On The Cell Type Colocalized With Pml And Eif3s6 In Nuclear Bodies Recruited To Retromer-Containing Endosomes Via Interaction With Magel2 Co-Localizes With P62/Sqstm1 And Tbk1 In Cytoplasmic Structures That Are Closely Associated With The Mitochondria
Alternative Antibody Names	Anti-Zinc Finger Protein Rfp antibody Anti-Ring Finger Protein 76 antibody Anti-Ret Finger Protein antibody Anti-Tripartite Motif-Containing Protein 27 antibody Anti-TRIM27 antibody Anti-RFP antibody Anti-RNF76 antibody

Information sourced from Uniprot.org

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