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> Anti-Rabbit IgG (H&L) (Rhodamine Conjugated) Secondary Antibody

Anti-Rabbit IgG (H&L) (Rhodamine Conjugated) Secondary Antibody

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Cat# ASR1424

Size : 2mg

Brand : Abcepta

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  • WB - Anti-Rabbit IgG (H&L) (Rhodamine Conjugated) Secondary Antibody ASR1424
    Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
  • WB - Anti-Rabbit IgG (H&L) (Rhodamine Conjugated) Secondary Antibody ASR1424
    Western Blot of Goat anti-Rabbit IgG Rhodamine Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
  • DB - Anti-Rabbit IgG (H&L) (Rhodamine Conjugated) Secondary Antibody ASR1424
    Dot Blot of Rhodamine Conjugated Goat-anti-Rabbit IgG. Antigen: Rabbit IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Goat-a-Rabbit IgG secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.
Product Information
Description Anti-RABBIT IgG (H&L) (GOAT) Antibody Rhodamine Conjugated
Host Goat
Conjugate Rhodamine (TRITC)
FP Value 3.6 moles Rhodamine (TRITC) per mole of IgG
Target Species Rabbit
Reactivity Rabbit
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note FLISA 1:10,000-1:50,000
IF Microscopy 1:1,000-1:5,000
FlowCytometry 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rabbit IgG whole molecule
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.