Anti-MOUSE IgG (H&L) (GOAT) Antibody
Cat# 610-1102
Size : 2mg
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for Mouse IgG (H&L) Antibody
Product Details
Goat Anti-Mouse IgG (H&L) Antibody - 610-1102
Goat anti-Mouse IgG Secondary Antibody, Mouse Secondary Antibody, GAM Antibody, Gt-a-Ms antibody, anti-mouse secondary, Goat anti Mouse IgG
Goat
IgG (H&L)
Polyclonal
IgG
Target Details
Mouse
Native Protein
Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
Application Details
ELISA, WB
LFA - View References
Anti-Mouse IgG affinity purified antibody is generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse IgG secondary antibody has been tested by ELISA and western blot and is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Formulation
2.19 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Wet Ice
Store Anti-Mouse Secondary Antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
Background
Anti-Mouse IgG Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This unconjugated secondary antibody has been validated and optimized yielding good sensitivity and reproducible results with Rockland's primary antibodies.
References (7)
Yuanxiang Huang et al. (2022). Edaravone Dexborneol Downregulates Neutrophil Extracellular Trap Expression and Ameliorates Blood-Brain Barrier Permeability in Acute Ischemic Stroke. Mediators Inflamm.
Applications
WB, IB, PCA PubMed
Latif MB et al. (2020). Relative contributions of the cGAS-STING and TLR3 signaling pathways to attenuation of herpes simplex virus 1 replication. J Virol.
Applications
WB, IB, PCA PubMed
Seemann S, Ernst M, Cimmaruta C, et al. (2020). Proteostasis regulators modulate proteasomal activity and gene expression to attenuate multiple phenotypes in Fabry disease. Biochem J.
Applications
WB, IB, PCA PubMed
Liu X et al. (2018). Acetate production from glucose and coupling to mitochondrial metabolism in mammals. Cell.
Applications
WB, IB, PCA PubMed
Dian Widiyanti et al. (2013). Development of immunochromatography-based methods for detection of leptospiral lipopolysaccharide antigen in urine Clin Vaccine Immunol.
Applications
Lateral Flow (LFA) PubMed
Waldschmidt TJ et al. (2002). Abnormal T cell-dependent B-cell responses in SCID mice receiving allogeneic bone marrow in utero. Severe combined immune deficiency. Blood.
Applications
E, EIA PubMed
Corstjens P. et al. (2001). Use of up-converting phosphor reporters in lateral-flow assays to detect specific nucleic acid sequences: a rapid, sensitive DNA test to identify human papillomavirus type 16 infection. Clin Chem.
Applications
Lateral Flow (LFA) PubMed
Related Protocols
Adherent Cell Lysis Protocol
Biotin Binding Assay Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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