Anti-LC3 (Human) pAb
Cat# PM036
Size : 100ul
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Please Note: MBL International will be shutting down its operations effective December 31, 2024. Distribution of MBL products in the United States will be transferred to Cosmo Bio US on January 1st while European Distributors will remain unchanged. For any US inquiries regarding orders or support during this transition, reach out to Cosmo Bio: https://www.cosmobiousa.com/. For Non-US inquiries reach out to MBL in Japan: https://www.mblbio.com/.
This LC3 antibody is validated for multiple applications (WB, IHC, ICC and IP) and isoform detection. PM036 has more than 1,000 citations where it used to generate data showing autophagy in research papers. (Saitoh T et al. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature 456, 264-8 (2008)). This is a polyclonal antibody that is raised in rabbit and is reactive with human, hamster, mouse, rat.
| Target | LC3 |
|---|---|
| Product Type | Antibody |
| Application | FCM, ICC, IHC, IP, WB |
| Clonality | Polyclonal |
| Conjugate | Unlabeled |
| Isotype | IgG |
| Immunogen | Recombinant human LC3 (MAP1LC3B :1-120 a.a.) |
| Host Species | Rabbit |
| Species Reactivity | Human, Hamster, Mouse, Rat |
| Formulation | PBS/50% glycerol, pH 7.2 |
| Research Area | Autophagy |
| Description/Background | Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect forms of LC3 (MAP1LC3A, B, C).</p> |
| Storage Temperature | -20°C |
| Protocols | FCM, ICC, IHC, IP, WB |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |