This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This APC conjugate is stable when stored at 28°C. Do not freeze.

Next Day 28°C

Red Laser (650 nm)

Clone 1a3 recognizes a monomorphic epitope on human HLADQ1. It does not crossreact with HLADR or HLADP.

HLADQ antibody, clone 1a3, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen DQ isotype (HLADQ). MHC class II is constitutively expressed on human professional antigenpresenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation². HLADQ consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain³ encoded by the HLADQA1 and HLADQB1 genes, respectively, located in the HLA complex of chromosome 6. The Nterminal α1 and β1 domains form the antigenbinding groove, which binds 1325 aa peptides derived from exogenous antigens⁴. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptideMHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling⁵. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation⁶ and can transduce signals into T cells, enhancing T cell proliferation and activity⁷. Specific alleles of HLADQ are associated with autoimmune diseases, including celiac disease⁸ and type 1 diabetes⁹, and graftversushost disease¹⁰.

HLADQ is expressed on antigenpresenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells.

CD3/TCR, CD8

1. Shookster L, et al. (1987) Hum Immunol. 20(1):5970
2. Holling TM, Schooten E, van Den Elsen PJ. (2004) Hum Immunol. 65(4):28290
3. Mitaksov V, Fremont DH. (2006) J Biol Chem. 281(15):1061825
4. Wieczorek M, et al. (2017) Front Immunol. 8:292
5. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):1691616921
6. Barnaba V, et al (1994) Eur J Immunol. 24(1):715
7. Di Rosa F, et al. (1993) Hum Immunol. 38(4):25160
8. Castaño L, et al. (2004) J Pediatr Gastroenterol Nutr. 39:80–84
9. Cucca F, et al. (1993) Hum Immunol. 37:85 –94
10. Petersdorf EW, (1996) Proc Natl Acad Sci USA. 93(26):1535863