Anti-Human HLA-DP (MHC Class II, Clone B7/21) Monomorphic - Biotin

This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Functional grade preclinical antibodies may be stored sterile as received at 28°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ 70°C. Avoid Repeated Freeze Thaw Cycles.

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Clone B7/21 recognizes a monomorphic epitope on human HLADP1, DP2, DP3, DP4, and DP5. It does not crossreact with HLADR or HLADQ.

This antiHLADP antibody is a recombinant chimeric version of the original clone B7/21 antibody. The variable domain sequences are identical to the original clone B7/21; however, the constant region sequences have been switched from mouse IgG3 which is prone to precipitation to human IgG1 to allow for easier downstream uses.

This Recombinant HLADP antibody, clone B7/21, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen DP isotype (HLADP). MHC class II is constitutively expressed on human professional antigenpresenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation¹. HLADP consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain² encoded by the HLADPA1 and HLADPB1 genes, respectively, located in the HLA complex of chromosome 6. The Nterminal α1 and β1 domains form the antigenbinding groove, which binds 1325 aa peptides derived from exogenous antigens³. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptideMHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling⁴. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation⁵ and can transduce signals into T cells, enhancing T cell proliferation and activity⁶. High HLADP expression is associated with an increased risk of graftversushost disease⁷. Specific alleles of HLADP are associated with autoimmune diseases, including rheumatoid arthritis⁸.

HLADP is expressed on antigenpresenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells.

1. Holling TM, et al. (2004) Hum Immunol. 65(4):28290.
2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):1061825.
3. Wieczorek M, et al. (2017) Front Immunol. 8:292.
4. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):1691616921.
5. Barnaba V, et al. (1994) Eur J Immunol. 24(1):715.
6. Di Rosa F, et al. (1993) Hum Immunol. 38(4):25160.
7. Petersdorf EW, et al. (2015) N Engl J Med. 373(7):599609.
8. Raychaudhuri S, et al. (2012) Nat Genet. 44(3):2916.