Anti-Human HLA-DP (MHC Class II) Monomorphic - Purified in vivo GOLD™ Functional Grade

Mouse IgG3

Unknown

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multistep process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 28°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ 70°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 28°C

Clone B7/21 recognizes a monomorphic epitope on human HLADP1, DP2, DP3, DP4, and DP5. It does not crossreact with HLADR or HLADQ.

HLADP antibody, clone B7/21, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen DP isotype (HLADP). MHC class II is constitutively expressed on human professional antigenpresenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation¹. HLADP consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain² encoded by the HLADPA1 and HLADPB1 genes, respectively, located in the HLA complex of chromosome 6. The Nterminal α1 and β1 domains form the antigenbinding groove, which binds 1325 aa peptides derived from exogenous antigens³. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptideMHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling⁴. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation⁵ and can transduce signals into T cells, enhancing T cell proliferation and activity⁶. High HLADP expression is associated with an increased risk of graftversushost disease⁷. Specific alleles of HLADP are associated with autoimmune diseases, including rheumatoid arthritis⁸.

HLADP is expressed on antigenpresenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells.

1. Holling TM, et al. (2004) Hum Immunol. 65(4):28290.
2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):1061825.
3. Wieczorek M, et al. (2017) Front Immunol. 8:292.
4. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):1691616921.
5. Barnaba V, et al. (1994) Eur J Immunol. 24(1):715.
6. Di Rosa F, et al. (1993) Hum Immunol. 38(4):25160.
7. Petersdorf EW, et al. (2015) N Engl J Med. 373(7):599609.
8. Raychaudhuri S, et al. (2012) Nat Genet. 44(3):2916.