Anti-CD20 (MS4A1, B-Lymphocyte Antigen CD20, B1, Bp35, CD20 Antigen, CD20 Receptor, LEU- 16, CVID5) (PerCP/Cy5.5) Monoclonal Antibody

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Cat# 367057-25T

Size : 25Tests

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Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

367057 CD20 (MS4A1, B-Lymphocyte Antigen CD20, B1, Bp35, CD20 Antigen, CD20 Receptor, LEU- 16, CVID5) (PerCP/Cy5.5)

Clone Type

Polyclonal

Host

mouse

Source

human

Swiss Prot

P11837

Isotype

IgG2b,k

Grade

Highly Purified

Applications

Crossreactivity

Accession #

NM_152866.3

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

CD20, a 33-36kD non-glycosylated type 1 transmembrane protein, is expressed by developing, resting and mature B cells, some follicular dendritic cells, and a small subset of mature T cells. B cell CD20 expression is lost upon differentiation into plasma cells. Functionally, CD20 activation contributes to B cell activation, proliferation, and differentiation.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. PerCP conjugates are sensitive to light.

Applications

Product Type: Mab|Isotype: IgG2b,k|Clone No: 2H7|Host: mouse|Source: human|Concentration: ~0.05mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with PerCP/Cy5.5. |Purity: Highly Purified|Immunogen: Human tonsillar B cells|Specificity: Recognizes the large extracellular loop of human CD20, mapped to the peptide sequence YNCEPANPSEKNSPST. Species Crossreactivity: Baboon, Cynomolgus monkey and Rhesus||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human tonsillar B cells

Form

Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with PerCP/Cy5.5.

Purity

Highly Purified

Specificity

Recognizes the large extracellular loop of human CD20, mapped to the peptide sequence YNCEPANPSEKNSPST. Species Crossreactivity: Baboon, Cynomolgus monkey and Rhesus

References

1. Clark EA, et al. 1985. Proc Natl Acad Sci USA. 82:1766. 2. Ledbetter JA & Clark EA. 1986. Human Immunol. 15:30. 3. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York. 4. Knapp W, et al. 1989 Leucocyte Typing IV. Oxford University Press. New York. 5. Schlossman S, et al. 1995 Leucocyte Typing V. Oxford University Press. New York.