Reagents and instruments for immunology, cell biology and molecular biology.
 
> Annexin V-FITC/PI Apoptosis Kit

Annexin V-FITC/PI Apoptosis Kit

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Cat# E-CK-A211-100

Size : 100Assays

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Detection Principle Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.
Detection Method Fluorometric method, Flow cytometry
Sample Type Cell samples
Assay Time 40 min
Detection Instrument Flow Cytometer
Dye Type FITC
Ex/Em 490/530
Channel Set FITC
Other Reagents Required PBS(E-BC-R187), Deionized water
Storage This product can be stored at 2-8°C for 12 months with shading light.
Expiration date 12 months
Shipping Ice bag
Cat.No. Product Name Sizes
E-CK-A111 Annexin V-FITC Reagent 500Tests , 200Tests , 100Tests , 50Tests
E-CK-A151 Annexin V Binding Buffer (10×) 50mL , 10mL
E-CK-A212 Annexin V-FITC/7-AAD Apoptosis Kit 200Assays , 100Assays , 50Assays , 20Assays
E-CK-A252 Annexin V-FITC/DAPI Apoptosis Kit 200Assays , 100Assays , 50Assays , 20Assays
E-CK-A301 Mitochondrial Membrane Potential Assay Kit (with JC-1) 100Assays , 50Assays , 20Assays
E-CK-A320 One-step TUNEL In Situ Apoptosis Kit (Green, FITC) 100Assays , 50Assays , 20Assays
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