AMPK alpha 2 (Ser173) Antibody
Cat# bs-5575R-20ul
Size : 20ul
AMPK alpha 2 (Ser173) Antibody
Applications
Reactivity
Predicted Reactivity
Overview | |
Catalog # | bs-5575R |
Product Name | AMPK alpha 2 (Ser173) Antibody |
Applications | WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) |
Specificity | Based on the high protein similarity this antibody will bind AMPK alpha 1 when phorphorylated at Ser184 and AMPK alpha 2 when phosphrylated at Ser173. |
Reactivity | Human, Mouse, Rat |
Predicted Reactivity | Cow, Pig, Horse, Rabbit |
Specifications | |
Conjugation | Unconjugated |
Host | Rabbit |
Source | KLH conjugated synthetic phosphopeptide derived from human PRKAA2 around the phosphorylation site of Ser173 |
Modification Site | Ser173 |
Clonality | Polyclonal |
Isotype | IgG |
Concentration | 1ug/ul |
Purification | Purified by Protein A. |
Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Target | |
Gene ID | 55635562 |
Subcellular location | Cytoplasm, Nucleus |
Synonyms | PRKAA2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; AMPK; AMPK2; PRKAA1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1 |
Background | The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008] |
Application Dilution | |
WB | 1:300-5000 |
ELISA | 1:500-1000 |
FCM | 1:20-100 |
IHC-P | 1:200-400 |
IHC-F | 1:100-500 |
IF(IHC-P) | 1:50-200 |
IF(IHC-F) | 1:50-200 |
IF(ICC) | 1:50-200 |