Reagents and instruments for immunology, cell biology and molecular biology.
 
> AM1-43

AM1-43

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Cat# 70024

Size : 1MG

Brand : Biotium

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AM1-43
AM1-43 (Equivalent to FM®1-43FX) - Image 2
AM1-43 (Equivalent to FM®1-43FX) - Image 3
Probe cellular localization

Membrane/vesicular
For live or fixed cells

For live/intact cells
Assay type/options

Real-time imaging
Fixation options

Fix after staining (formaldehyde), Permeabilize after staining
Colors

Green
Excitation/Emission

480/598 nm (in membranes)
Protocols
SDS
Supporting Documents

Product Description

AM1-43 (equivalent to FM®1-43FX) is a fixable, activity-dependent fluorescent nerve terminal probe. AM1-43 functions similarly to SynaptoGreen™ C4 (70020), but with the additional property of being fixable. Thus, AM1-43 is a useful tool for synapse studies where subsequent fluorescent immunochemistry is desired. AM1-43 and FM®1-43FX have identical excitation and emission wavelengths. Also see AM1-44 (catalog no. 70038), which is similar to AM1-43, but has longer lipophilic tails, resulting in better fixability.

  • Ex/Em: 510/625 nm (in MeOH); 480/598 nm (in membranes)
  • Red solid soluble in water
  • C29H49Cl3N4
  • MW: 559.5


Nerve Terminal Dyes and Kits

Product Catalog No. Features
SynaptoGreen™ Dyes
(Ex/Em ~480/660 nm in membranes)		 70020... 70053 View full list of SynaptoGreen™ Dyes
SynaptoRed Dyes™
(Ex/Em ~510/750 nm in membranes)		 70021... 70050 View full list of SynaptoRed™ Dyes
Background Reducers
ADVASEP-7 70029 Sulfonated cyclodextrin that aids in removal of free dye during washes
SCAS 70037 Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7
Sulforhodamine 101 80101 Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits
Nerve Terminal Staining Kit I 70030 Includes SynaptoGreen™ C4 and ADVASEP-7
Nerve Terminal Staining Kit II (A) 70031 Includes AM1-43 and ADVASEP-7
Nerve Terminal Staining Kit II (B) 70031-1 Includes AM1-43 and SCAS
Nerve Terminal Staining Kit III 70032 Includes SynaptoGreen™ C4 and Sulforhodamine 101
Nerve Terminal Staining Kit V 70034 Includes SynaptoRed™ C2 and ADVASEP-7
m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.

References

1. PLoS One, 5(8), e12386.

2. Anat Cell Biol 44(4), 274 (2011).

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