Agarose for DNA Fragments 50-1000bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels.||Manufactured specifically for primer size fragments in the range of 50-1000 base pairs; exhibits exceptionally high transparency even at high concentrations (4.5%).||Applications:|• Analytical DNA Gels ≤ 1000bp|• PCR products|• Restriction Enzyme digestion products|• Mutation Analysis|• Blotting Assays ≤ 600bp|• For use with all buffer systems||Recommended Usage (1X TAE):|Concentration Molecular Weight|1.8% 500-1000 bp |3.0% 150-600 bp|4.5% 50-350 bp|Note: A 3% agarose gel is equivalent to 8% polyacrylamide||Source:|Gelidium||Form:|White, homogeeous free flowing powder||EEO:|≤0.13||Clarity (1.5%, NTU):|~4||Absorbance (430nm, 1.5%):|≤0.040||pH (1.5%, H2O, Solution):|6-7||pH (1.5%, H2O, Gel):|5.0-7.5||Ash:|≤0.35%||Sulfate:|≤0.1%||Melting Point (3%):|≤78°C||Gel Point (3%):|≤35°C||Gel Strength (1.5%):|≥750g/cm2||Gel Strength (3%):|≥1500g/cm2||RNase:|None Detected||DNase:|None Detected||Quality Control:|Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.||Functional and Molecular Biology Testing:|To Pass Test|• Comparative assay of different size DNA fragments|• Southern Blot (125-23,000bp)|• Background Fluorescence (TAE/TE, EtBr): |• DNA Binding (TAE, EtBr) |• Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)||Storage and Stability: |Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.||Important Note: |This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. ||Toxicity and Hazards: |All products should be handled by qualified personnel only, trained in laboratory procedures.