10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride) (4 x 1 liter)
Cat# MB-008-4000
Size : 4x1L
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for 10X PBS pH 7.2
Product Details
10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride) - MB-008
Phosphate buffered saline, Phosphate buffered solution, PBS, 10X PBS
Target Details
10X PBS buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Application Details
FC, IF, Other - View References
This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium Chloride, pH 7.2 prepared in highly polished pharmaceutical grade water (WFI).
Formulation
10X N/A
See application note.
None
None
Shipping & Handling
Ambient
Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.
Expiration date is six (6) months from date of receipt.
Background
Phosphate buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.
References (32)
Galbraith MD et al. (2023). Multidimensional definition of the interferonopathy of Down syndrome and its response to JAK inhibition. Sci Adv.
Applications
FC, FACS, FLOW PubMed
Gihring A et al. (2023). Influence of bariatric surgery on the peripheral blood immune system of female patients with morbid obesity revealed by high-dimensional mass cytometry. Front Immunol.
Applications
FC, FACS, FLOW PubMed
Mohamad SF et al. (2023). Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype. Methods Mol Biol.
Applications
FC, FACS, FLOW PubMed
Sperber HS et al. (2023). The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency. Cell Rep.
Applications
FC, FACS, FLOW PubMed
Su C et al. (2022). 3D chromatin maps of the human pancreas reveal lineage-specific regulatory architecture of T2D risk. Cell Metab.
Applications
RNA seq, qPCR, Reverse Transcription, RT-PCR Analysis PubMed
Lança T et al. (2022). IRF8 deficiency induces the transcriptional, functional, and epigenetic reprogramming of cDC1 into the cDC2 lineage. Immunity.
Applications
FC, FACS, FLOW; IF, Confocal Microscopy PubMed
Agosto-Burgos C et al. (2022). The frequency of Treg subsets distinguishes disease activity in ANCA vasculitis. Clin Transl Immunology.
Applications
FC, FACS, FLOW PubMed
Neidleman J et al. (2021). Distinctive features of SARS-CoV-2-specific T cells predict recovery from severe COVID-19. medRxiv.
Applications
Other PubMed
Jaeger N et al. (2021). Single-cell analyses of Crohn's disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions. Nat Commun.
Applications
FC, FACS, FLOW PubMed
Sahaf, B et al. (2021). Immune Profiling Mass Cytometry Assay Harmonization: Multicenter Experience from CIMAC-CIDC. Clinical Cancer Research : An Official Journal of the American Association for Cancer Research
Applications
Buffer PubMed
Henrick BM et al. (2021). Bifidobacteria-mediated immune system imprinting early in life. Cell.
Applications
FC, FACS, FLOW PubMed
Xie G et al. (2021). Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4+ T cell subsets in vivo. Cell Rep.
Applications
FC, FACS, FLOW PubMed
Vasudevan S et al. (2020). Lower PDL1, PDL2, and AXL Expression on Lung Myeloid Cells Suggests Inflammatory Bias in Smoking and Chronic Obstructive Pulmonary Disease. Am J Respir Cell Mol Biol.
Applications
FC, FACS, FLOW PubMed
Lakshmikanth T et al. (2020). Human immune system variation during 1 year. Cell Rep.
Applications
FC, FACS, FLOW PubMed
Sahaf B et al. (2020). High-parameter immune profiling with CyTOF. Methods Mol Biol.
Applications
FC, FACS, FLOW PubMed
Colomb F et al. (2020). Sialyl-LewisX Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy. Cell Rep.
Applications
FC, FACS, FLOW PubMed
Rodriguez L et al. (2020). Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19 Cell Rep Med.
Applications
FC, FACS, FLOW PubMed
Neidleman J et al. (2020). SARS-CoV-2-specific T cells exhibit phenotypic features of helper function, lack of terminal differentiation, and high proliferation potential. Cell Rep Med.
Applications
FC, FACS, FLOW PubMed
Vendrame E et al. (2020). Profiling of the Human Natural Killer Cell Receptor-Ligand Repertoire. J Vis Exp.
Applications
FC, FACS, FLOW PubMed
Fenton TM et al. (2020). Immune profiling of human gut-associated lymphoid tissue identifies a role for isolated lymphoid follicles in priming of region-specific immunity. Immunity.
Applications
FC, FACS, FLOW; IF, Confocal Microscopy PubMed
Waugh KA et al. (2019). Mass cytometry reveals global immune remodeling with multi-lineage hypersensitivity to type I interferon in Down syndrome. Cell Rep.
Applications
FC, FACS, FLOW PubMed
Cella M et al. (2019). Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues. Nat Immunol.
Applications
FC, FACS, FLOW PubMed
Collins PL et al. (2019). Gene regulatory programs conferring phenotypic identities to human NK cells. Cell.
Applications
FC, FACS, FLOW PubMed
Cella, M et al. (2019). Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues. Nature Immunology
Applications
Buffer; FC, FACS, FLOW PubMed
Olin A et al. (2018). Stereotypic immune system development in newborn children. Cell.
Applications
FC, FACS, FLOW PubMed
Leipold MD et al. (2018). Comparison of CyTOF assays across sites: Results of a six-center pilot study. J Immunol Methods.
Applications
FC, FACS, FLOW PubMed
Subrahmanyam PB et al. (2017). Cytof measurement of immunocompetence across major immune cell types. Curr Protoc Cytom.
Applications
FC, FACS, FLOW PubMed
Leipold MD et al. (2015). Phenotyping of live human PBMC using CyTOFTM mass cytometry. Bio Protoc.
Applications
FC, FACS, FLOW PubMed
Lin D et al. (2015). Intracellular cytokine staining on PBMCs Using CyTOF™ mass cytometry. Bio Protoc.
Applications
FC, FACS, FLOW PubMed
Fernandez R et al. (2015). Cytokine-stimulated phosphoflow of PBMC using CyTOF mass cytometry. Bio Protoc.
Applications
FC, FACS, FLOW PubMed
Fernandez, R et al. (2015). Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass Cytometry. Bio-Protocol
Applications
FC, FACS, FLOW; Buffer PubMed
JENNIFER BERGER (2012). GENE EXPRESSION PATTERNS OF GAMMAHERPESVIRUSES. Olaf College
Applications
IF, Confocal Microscopy Related Protocols
Flow Cytometry (FC) Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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