PCR enhancers are specialized reagents used to improve PCR performance and increase sensitivity. These enhancers can improve the amplification of difficult targets and reduce reaction inhibition. They can also enhance the specificity of PCR by promoting the amplification of the desired product while suppressing the amplification of non-specific products.

PCR enhancers can be added to the PCR reaction mix in various concentrations, depending on the specific enhancer and the application. However, it is important to optimize the concentration of the enhancer, as too high a concentration can inhibit the PCR reaction and lead to poor results.