Total Carbohydrate Colorimetric Assay Kit
Cat# E-BC-K755-M-48T
Size : 48T
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| Detection Principle | Carbohydrate is one of the important components of various bodies, and is also the main raw material and storage material of metabolism. Total carbohydrate mainly refers to reducing glucose, fructose, pentose, lactose, maltose, sucrose that can be hydrolyzed to reducing monosaccharides under determined conditions, and possibly partially hydrolyzed starch. The total carbohydrate was hydrolyzed to reducing sugar, which was reduced to amino compounds after co-heating with 3,5-dinitrosalicylic acid under alkaline conditions. The amount of reducing sugar was proportional to the color of the reddish brown substance, and the total carbohydrate content in the sample was determined by this method. |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-550 nm, optimum wavelength: 540 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Plant Physiology Series |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.036 mg/mL |
| Detection Range | 0.036-1.0 mg/mL |
| Precision | inter-assay CV: 2.1-5.3% | intra-assay CV: 1.0-2.9% |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Wheat tissue homogenate | 30-60 |
| 10% Paddy tissue homogenate | 30-60 |
| 10% Corn tissue homogenate | 30-60 |
| 10% Pumpkin tissue homogenate | 30-50 |
| 10% Mouse liver tissue homogenate | 3-10 |
| Human serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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